Tag

Part:BBa_K3032003

Designed by: Denis Baronas   Group: iGEM19_Vilnius-Lithuania   (2019-10-12)


Light-inducible protein degradation tag (162AtLOV2)

A light-inducible protein degradation tag, made by fusing LOV2 domain from protein phototropin 1 of Arabidopsis thaliana with protein degradation tag of Mesoplasma florum (mf-Lon). Such degradation tag is recognisable by mf-Lon protease when cells are illuminated with blue light.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 400


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